Keratinase Production by Bacillus sp. MD24 in Sub-merge and Solid State Fermentation

Chicken feathers are valuable solid waste that mainly contains insoluble proteins called keratin. Naturally, keratin is degraded by microbes produce a specific protease keratinase. Many reports have been addressed to study Bacillus species as keratin-degrading microbes. However, most reported works were using pathogenic soil Bacillus. Our work has concentrated on keratinase produced bacterium, sp. MD24 and we confirmed it non-pathogenic bacterium. This report describes the different profiles of fermentation between sub-merged (SmF) solid-state (SSF) chicken sole carbon nitrogen source. Keratinase production weight loss followed for 10 consecutive days, molecular was investigated, type concentration 18 amino acids determined LC-MS/MS after days seek its potential acid source, protein by-product measured. The results showed well in SmF better compared SSF with optimum times. Surprisingly, L-arginine dominant hydrolysate which contained 42.06% total made organism production. As relatively low both conditions showing effective utilization matter energy consequently, cell-free should be applied degrade into materials.